FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (24): 248-251.doi: 10.7506/spkx1002-6630-201124053

• Analysis & Detection • Previous Articles     Next Articles

GPC Separation of Procyanidin Oligomers from Sorghum Episperm

LI Ying1,ZHANG Xiao-pan2,YE Han-ying1   

  1. (1. Department of Environmental and Biochemical Engineering, Wuhan Vocational College of Software and Engineering, Wuhan 430205, China;2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Online:2011-12-25 Published:2011-12-29

Abstract: A method for the separation and composition analysis of procyanidins from sorghum episperm was established in this study. Sorghum episperm procyanidins (SPCs) were extracted with aqueous ethanol, primarily purified by ADS-17 macroporous resin adsorption, fractionated by Toyopearl HW-40 gel chromatography, and preliminarily identified by UV spectroscopy and HPLC with diode array detection (DAD). Fractions A, B, C, D, E and F were obtained as a result of Toyopearl HW-40 gel chromatographic separation. According to the results of UV spectroscopic analysis, fraction B was flavonoid, and all other fractions were procyanidin. Fractions C, D and F were identified by HPLC-DAD as procyanidin dimer B1, individual catechin and procyanidin oligomer, respectively. The method allowed good separation and analysis of procyanidins from sorghum episperm.

Key words: sorghum episperm, procyanidins, gel chromatography, high performance liquid chromatography (HPLC), isolation

CLC Number: