Identification of Water Buffalo Caseins by Liquid Chromatography and LTQ-Orbitrap Mass Spectroscopy

doi:10.7506/spkx1002-6630-201201019

Abstract

Abstract:

A rapid and efficient method to analyze the amino acid sequence of casein was presented using LTQ-Orbitrap mass spectroscopy coupled with liquid chromatography. In order to understand the amino acid sequence and substitution of cow, water buffalo and goat caseins, LTQ-Orbitrap mass spectroscopy coupled with liquid chromatography was used to analyze 4 major casein components, and their complete amino acid sequences were acquired by database searching. The amino acid substitutions of cow milk caseins were markedly lower than those of goat milk caseins. Thus, this study demonstrates that the amino acid sequences of cow milk caseins are more stable than those of goat milk caseins.

Key words: water buffalo milk, casein, amino acid sequence, casein structure, LTQ-Orbitrap mass spectrometry

CLC Number:

Q657.6

WANG Li-na，XU Ming-fang，CHENG Xi-fei，XIANG Ming-xia，LI Zi-chao，LI Yun-kai. Identification of Water Buffalo Caseins by Liquid Chromatography and LTQ-Orbitrap Mass Spectroscopy[J]. FOOD SCIENCE, 2012, 33(1): 98-102.

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