Screening and Identification of a High-Yield Neutral Phytase-Producing Strain and Characterization of Neutral Phytase

doi:10.7506/spkx1002-6630-201201024

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 120-124.doi: 10.7506/spkx1002-6630-201201024

• Bioengineering • Previous Articles Next Articles

Screening and Identification of a High-Yield Neutral Phytase-Producing Strain and Characterization of Neutral Phytase

WANG Tao，LI Wen，YUAN Pei-pei

College of Food (Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221111, China

Received:2011-02-21 Revised:2011-12-26 Online:2012-01-15 Published:2012-01-12
Contact: WANG Tao E-mail:wangtaoxz@xzit.edu.cn

Abstract

Abstract:

A high-yield neutral phytase-producing strain with an enzymatic activity of 12.52 U/mL was isolated from soil and initially identified as Agrobacterium radiobacter based on its morphological, physiological and biochemical characteristics as and 16S rDNA sequence. The optimum reaction temperature and pH of neutral phytase produced by the strain were 45 ℃ and 7.0, respectively. The relative activity of the enzyme was 80% after heating at 65 ℃ for 60 min, revealing that it was thermostable to some extent. The enzyme was stable at pH values in the range of 5.5－8.0. Its activity could be activated by Ba2+ but inhibited by Fe2+ , Mg2+, Zn2+ and Cu2+ .

Key words: neutral phytase, screening, identification, enzymatic properties

CLC Number:

S816.7
Q933

WANG Tao，LI Wen，YUAN Pei-pei. Screening and Identification of a High-Yield Neutral Phytase-Producing Strain and Characterization of Neutral Phytase[J]. FOOD SCIENCE, 2012, 33(1): 120-124.

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Screening and Identification of a High-Yield Neutral Phytase-Producing Strain and Characterization of Neutral Phytase

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