Extraction and Purification of Cellulase from Spent Mushroom Compost

doi:10.7506/spkx1002-6630-201201025

Abstract

Abstract:

Spent mushroom compost (SMC) was used to prepare industrial grade cellulase by solvent extraction, membrane
filtration and salting out and high-purity cellulase by further aqueous two-phase extraction. The results showed that crude
cellulase solution with an enzymatic activity of 102.64 U/mL was obtained after 3 h of extraction at 25 ℃ with an extraction solvent
composed of 0.2 mol/L acetic-acetate sodium buffer at pH 5.0 and 0.2% Triton X-100. Subsequent salting out at 60% (NH4)2SO4
saturation could recover 65.54% of the total cellulase activity, and industrial grade cellulase with an enzymatic activity of
2865.47 U/mL was obtained. After further aqueous two-phase extraction with a system made up of 22% polyethylene glycol
6000 (PEG6000) and 20% (NH4)2SO4 under the conditions of 7 mmol/L NaCl, pH 5.5 and normal temperature, the cellulase activity
and specific activity were 10208.46 U/mL and 106.62 U/mg, respectively and a total activity yield of 51.22% was achieve,
revealing a 12.85-fold increase when compared with that of crude enzyme solution. As a result, high-purity cellulase was
obtained.

Key words: spent mushroom compost(SMC), cellulose, aqueous two-phase extraction, extraction and purification

CLC Number:

TS209

DU Ning，XU Tian-tian，LI Xian-fu，XIA Zhi-hua，CHEN Jun. Extraction and Purification of Cellulase from Spent Mushroom Compost[J]. FOOD SCIENCE, 2012, 33(1): 125-130.

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