Purification and Cytotoxicity of C-Phycocyanin(C-PC) from Spirulina platensis and Its Tryptic Peptides

doi:10.7506/spkx1002-6630-201201027

Abstract

Abstract:

This paper deals with the separation, purification and cytotoxicity of C-phycocyanin (C-PC) from Spirulina platensis and its tryptic peptides. Repeated freezing and thawing coupled with ultrasonic treatment was used for disrupting the cell wall of Spirulina platensis. The purity (A620nm/A280nm) of C-PC was 2.19 after fractional precipitation by 28－55 g/100 mL (NH4)2SO4 and could reach 3.89 after further purification by sequential chromatography on hydroxylapatite (HA) column and Sephacryl S-200 HR gel column. The purified C-PC was hydrolyzed by trypsin at 40 ℃ for 60 min. Four peptides were obtained from the hydrolysate of C-PC by DEAE-Sepharose Fast Flow column chromatography. The cytotoxicity of C-PC and its hydrolysate as well as the 4 peptides on HeLa and 293T cells was evaluated by MTT assay. The results showed that the inhibition rates of peptide fractions I and IV, C-PC hydrolysate and C-PC on the growth of HeLa cells were 37.71%, 47.04%, 34.02% and 26.03%, respectively. Therefore, peptide fractions I and IV revealed obvious suppressive effect on the proliferation of cancer cells, while neither of them had cytotoxicity on 293T cells. Moreover, peptide fraction IV had the strongest tumor suppression activity, indicating a great potential to be developed as health-care products.

Key words: C-phycocyanin, purification, hydrolyzed peptide, cytotoxicity

CLC Number:

WANG Xue-qing，DENG Wei，YANG Jin-fang，MAO Yu-cong，SHI Zhong-ming. Purification and Cytotoxicity of C-Phycocyanin(C-PC) from Spirulina platensis and Its Tryptic Peptides[J]. FOOD SCIENCE, 2012, 33(1): 136-140.

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