A Comparative Study of Rapid Determination Methods for Biomass of Chlorella protothecoides CS-41

doi:10.7506/spkx1002-6630-201202056

Abstract

Abstract: Absorbance and optical density of Chlorella protothecoides CS-41 were measured by a spectrophotometer and a microplate spectrophotometer, respectively. Total cell number and dry biomass of C. protothecoides were evaluated by microscope and drying method. A good linear relationship between the absorbance/optical density and microscopic counting/biomass was established. The wavelength of 440 nm was chosen as the optimal wavelength for measuring absorbance/optical density of biomass. Through the comparison of both kinds of instruments, microplate spectrophotometer revealed more obvious linear relationship and optical density method could provide more convenience, efficiency and accuracy for the determination of biomass.

Key words: Chlorella protothecoides CS-4, biomass, absorbance, optical density, microscopic counting, dry weight

CLC Number:

Q949.21

CUI Yan, LI Mei-ya, SHI Chun-lei, SHI Xian-ming. A Comparative Study of Rapid Determination Methods for Biomass of Chlorella protothecoides CS-41[J]. FOOD SCIENCE, 2012, 33(2): 253-257.

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A Comparative Study of Rapid Determination Methods for Biomass of Chlorella protothecoides CS-41

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