FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (4): 66-70.doi: 10.7506/spkx1002-6630-201204014
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LIU Sheng-nan,WAN Xiao-chun,DU Xian-feng
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Published:
Abstract: An enzymatic method for the preparation of cello-oligosaccharides from Radix Puerariae fiber was developed, and HPLC was employed to determine the sugar composition of cello-oligosaccharide extracts. The effects of enzyme type and dose as well as hydrolysis time on cello-oligosaccharide composition and contents were explored. The results showed that β-glucanase was more suitable for the preparation of cello-oligosaccharides than cellulase. Hydrolysates consisting of mainly glucose, cellobiose and cellotriose were obtained using β-glucanase. The hydrolysate obtained after 2 h of hydrolysis with 3 U/g β-glucanase contained a high proportion of cellobiose and cellotriose and the yield of cello-oligosaccharides was 11.8 g/100 g under these conditions.
Key words: Radix Puerariae fiber, cello-oligosaccharides, β-glucanase
CLC Number:
TS241
LIU Sheng-nan,WAN Xiao-chun,DU Xian-feng. Enzymatic Preparation of Cello-oligosaccharides from Radix Puerariae Fiber[J]. FOOD SCIENCE, 2012, 33(4): 66-70.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201204014
https://www.spkx.net.cn/EN/Y2012/V33/I4/66