Abstract: Static-dynamic supercritical CO2 fluid extraction was carried out at 50 ℃ and 40 MPa for 1 h in each mode using 75% ethanol as the entrainer for extracting Bamboo polysaccharides from the leaves of Phyllostachys heterocycla, Pleioblastus amarus, Dendrocalamopsis oldhami, and Acidosasa edulis at an entrainer-to-material ratio of 3:1 (mL/g). The polysaccharide contents of the leaves of Phyllostachys heterocycla, Pleioblastus amarus, Dendrocalamopsis oldhami, and Acidosasa edulis, as calculated based on a dry matter basis, were 16.585, 12.804, 8.255 mg/g and 6.329 mg/g, respectively. The polysaccharide samples obtained were hydrolyzed with trifluoroacetic (TFA), acetylated and analyzed for monosaccharide composition by gas chromatography. Each polysaccharide contained mannose, rhamnose, galactose, glucose, arabinose, xylose and fucose. The predominant saccharide was glucose in D. oldhami, P.amarus and P. heterocycla and galactose in A. edulism. D. oldhami leaves contained 2.345 mg/g glucose and 1.432 mg/g and galactose, both of which were the most predominant monosaccharides in the four species of bamboo leaves.

Key words: supercritical fluid extraction, bamboo polysaccharides, gas chromatography, pre-column derivatization, monosaccharide composition