Ethanol Production by Fermentation of Corncob Hydrolysate with High Ratio of Hexose Prepared with Cellulase

doi:10.7506/spkx1002-6630-201207045

Abstract

Abstract: Corncobs were crushed into 1－2 cm3, moistened by spraying 15g/100mL sulfuric acid, extracted for the removal of pentose, and then subjected to three-step countercurrent enzymatic hydrolysis and extraction. Hydrolysate with a hexose content of 10.26 g/100 mL was obtained. The fermentation of the hydrolysate by Saccharomyces cerevisiae HF2.0 for ethanol production was investigated. Placktt-Burman (PB) design was used to analyze the effects of MgSO4, KH2PO4, CaCl2, soybean meal hydrolysate, wheat bran hydrolysate, and yeast mud added to the hydrolysate on ethanol production. MgSO4, KH2PO4 and soybean meal hydrolysate were identified as the main influential factors. As determined by steepest ascent tests and central composite design, the best fermentation results were obtained when the concentrations of MgSO4, KH2PO4 and soybean meal hydrolysate were 0.045, 0.135 g/100 mL and 7.34 g/100 mL, respectively, resulting in an ethanol yield of 88.64 g/L.

Key words: hexose, corn cob, enzymatic hydrolysis, ethanol fermentation

CLC Number:

Q815

ZHAOHui，WANGHai-ji，WANGWei，LINGHong-zhi，PINGWen-xiang. Ethanol Production by Fermentation of Corncob Hydrolysate with High Ratio of Hexose Prepared with Cellulase[J]. FOOD SCIENCE, 2012, 33(7): 215-218.

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Ethanol Production by Fermentation of Corncob Hydrolysate with High Ratio of Hexose Prepared with Cellulase

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