Interaction of Epigallocatechin-3-gallate with Porcine Pancreas Lipase

doi:10.7506/spkx1002-6630-201307005

Abstract

Abstract: The interaction between epigallocatechin-3-gallate (EGCG) and porcine pancreas lipase (PPL) was studied by fluorescence spectroscopy, and the mechanism was investigated according to molecular docking. Results showed that EGCG could quench PPL fluorescence and inhibit its catalytic activity, while the inhibition was weakened in the presence of Ca2+, Mg2+, Cu2+ or Fe2+. Molecular docking suggested that EGCG might be bound to PPL through hydrophobicity, hydrogen bonds and Pi-Pi interaction, and blocked the entry of substrate into the active center, thus inhibiting the catalytic activity of PPL.

Key words: epigallocatechin-3-gallate, porcine pancreas lipase, fluorescence quenching, molecular docking

CLC Number:

TS201.2

FAN Zhi-fei，ZENG Wei-cai，DAI Ji-ling，HE Qiang*. Interaction of Epigallocatechin-3-gallate with Porcine Pancreas Lipase[J]. FOOD SCIENCE, 2013, 34(7): 20-23.

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