Effect of Endogeneous Proteinases on Autolysis of Squid Hepatopancreas

doi:10.7506/spkx1002-6630-201307046

Abstract

Abstract: Functional properties of endogeneous proteinases from squid hepatopancreas and their role in the autolysis of squid hepatopancreas were studied. Proteinases activity was analyzed by determining TCA soluble peptides using azocasein as a substrate. The results indicated that the optimal pH and temperature were 5.0 and 40 ℃ for crude endogeneous proteinases, respectively. The enzyme activity remained stable over the pH range of 3.0 to 8.0. The autolysis inhibitory effect of E-64 proved that the cysteine proteinase played a dominant role in autolysis of squid hepatopancreas. Moreover, other endogeneous enzymes such as serine proteinase and metallo proteinase also demonstrated varying impacts on the autolysis.

Key words: squid hepatopancreas, proteinase, autolysis

CLC Number:

TS254.9

CHEN Zhao，TIAN Yuan-yong*，MA Chun，ZHU Bei-wei. Effect of Endogeneous Proteinases on Autolysis of Squid Hepatopancreas[J]. FOOD SCIENCE, 2013, 34(7): 223-226.

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