FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (7): 231-233.doi: 10.7506/spkx1002-6630-201307048

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Mutation Breeding of a High Polygalacturonase-Producing Aspergillus niger Strain

ZHANG Yuan-yuan,HAO Lin*,WANG Qian   

  1. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China
  • Received:2011-12-24 Revised:2013-02-26 Online:2013-04-15 Published:2013-03-20
  • Contact: HAO Lin E-mail:haolinsxnd@126.com

Abstract: Aspergillus niger strain SH312-26 was isolated by apparent circle method from strain SH312. A mutant strain named as SH312-26-19 was obtained by UV radiation using SH312-26 the original strain. The polygalacturonase activity of SH312-26-19 was 113.68 kU/mL, a 1.44-fold increase than that of strain SH-312-26. The pectin esterase activity was 14.34 kU/mL, a 0.14-fold increase than that of the original strain. After 5 passages on slant culture medium, the mutant remained genetically stable.

Key words: polygalacturonase, mutation breeding, selection

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