Determination of Carbofuran and Carbaryl Residues in Fruits and Vegetables by Nonaqueous Micellar Electrokinetic Capillary Chromatography with Online Enrichment

doi:10.7506/spkx1002-6630-201308037

Abstract

Abstract: A method of nonaqueous micellar electrokinetic capillary chromatography with online enrichment was developed to determine carbofuran and carbaryl residues in fruits and vegetables. The effects of buffer composition, apparent pH, applied voltage, and injection time were systematically discussed. The optimized buffer was composed of 100 mmol/L sodium dodecyl sulfate, 30 mmol/L sodium acetate, and formamide-acetonitrile (85:15, V/V), and its apparent pH was adjusted to 9.4. The applied voltage was 20 kV and the sample injection time was 150 s. Under these optimal conditions, peak enrichment factor was 678 and 758; detection limit was 31 μg/L and 7.9 μg/L; RSD was 2.47% and 1.76%; average recovery rates of spiked samples were 80.4%–96.2% and 92.5%–104.1% for carbofuran and carbaryl residues, respectively. Therefore, this method is of high sensitivity, good repeatability and can be used in the determination of both pesticide residues in fruits and vegetables.

Key words: nonaqueous micellar electrokinetic capillary chromatography；carbofuran；carbaryl residue, formamide, enrichment

CLC Number:

O657.8

TONG Ming-zhu，CHEN Guan-hua*，WU Chuan-qin，GUO Dong-shan，FANG Rou，ZHANG Li. Determination of Carbofuran and Carbaryl Residues in Fruits and Vegetables by Nonaqueous Micellar Electrokinetic Capillary Chromatography with Online Enrichment[J]. FOOD SCIENCE, 2013, 34(8): 176-181.

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