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Screening and Identification of Antimicrobial Protein-producing Strain

FAN Chen1,GAO Zhao-jian1,*,CHEN Hong-wei1,JU Min-you2,ZHANG Gui-ying3,CHEN Shang-long1   

  1. 1. College of Food(Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China;
    2. Jiangsu Taihe Food Co. Ltd., Suining 221200, China;3. Wanjia Animal Husbandry Science and
    Technology Development Co. Ltd., Tongshan 221100, China
  • Online:2013-07-15 Published:2013-06-28
  • Contact: GAO Zhao-jian

Abstract:

A new strain, ZHL24, capable of producing broad-spectrum antimicrobial protein was isolated from soil.
The antibacterial protein produced by the strain showed a high inhibitory activity towards many pathogenic bacteria and
fungi such as Escherichia coli, Mirococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Candida albicans and
Rhizopus stolonifer. This new strain was identified as Bacillus subtilis based on physiological characteristics, biochemical
characteristics and 16S rDNA sequence. The antimicrobial protein was purified to homogeneity through ammonium sulfate
precipitation, ion exchange chromatography (DEAE-Sepharose FF) and gel filtration (Sephadex G-15). SDS-PAGE revealed
a single band at the molecular mass of 26.2 kD. Amino acid composition analysis showed that the antibacterial protein
contained 14 kinds of amino acids.

Key words: broad-spectrum antimicrobial protein, Bacillus subtilis, screening and identification, purification

CLC Number: