Abstract:

Cyanidin-3-O-β-glucoside (Cy-3-g)-rich foods have been reported to ameliorate the onset of insulin resistance,
but the underlying mechanism has remained unclear. PTEN (phosphatase and tensin homologue deleted on chromosome
10) plays a negative role in insulin signaling-PI3K (phosphatidylinositol 3-kinase)/Akt (protein kinase B), and its
inhibition improves insulin sensitivity. In order to gain insight into the effect of Cy-3-g anthocyanin on insulin sensitivity
and the role of PTEN, KKAy mice were fed AIN-93G diet supplemented with or without Cy-3-g (1 g/kg diet) for 12
weeks. Our results demonstrated that Cy-3-g ameliorated insulin sensitivity, and inhibited the PTEN expression in liver,
muscle and viscera adipose tissues following the activation of Akt (Ser473) in mice. These findings provided evidence for
the role of PTEN in PI3K/Akt (Ser473) pathway, thus suggesting that the possible mechanism may be the contribution of
Cy-3-g to insulin signaling.

Key words: cyanidin-3-O-&beta, -glucoside；insulin resistance；PTEN；KKAy mice