Abstract:

The best macroporous resin for purification of banana peel flavonoid (BPF) was selected from X-5, AB-8, H103 and
D-101 by static adsorption and desorption capacities. H103 was identified as the best resin for BPF purification. The adsorption
equilibrium was reached in about 3 h and the maximum adsorption quantity for BPF was 3.66 mg/g, while the time required to
reach desorption equilibrium was about 2.5 h, yielding a desorption percentage of 89.11%. The optimal conditions for purifying
BPF by dynamic adsorption and desorption on H103 were determined as 7.0, 2.0 mL/min, 3.0 mL/min and 75% ethanol for pH
sample loading flow rate, desorption flow rate and desorbent, respectively. Under these conditions, the recovery and purity of
BPF were 72.50% and 73%, respectively. BPF could inhibit the growth of Candida albicans, Escherichia coli, Bacillus subtilis,
and purified BPF had better antimicrobial activity.

Key words: banana peel flavonoid, macroporous resin, purification, antimicrobial activity