Abstract:

Fucoxanthin was extracted from Laminaria japonica with composite enzyme (cellulosase:pectinase = 1:1).
Single factor method and orthogonal array design were adopted to establish the optimum extraction conditions: enzyme
dosage 0.30%, pH 5.0, treatment time 80 min and temperature 50 ℃. Under these conditions, the yield of fucoxanthin was
18.30 mg/100 g (wet weight), an increase of 26.5% and 15%－20% compared with that observed with traditional organic
solvent extraction and enzymatic extraction with a single enzyme, respectively. The reducing power and superoxide anion
radical scavenging activity of the fucoxanthin from Laminaria japonica against increased with concentration within the
studied range, and the EC50 value against superoxide anion free radical was 1.27 mg/mL.

Key words: Laminaria japonica, fucoxanthin, complex enzyme, antioxidant activity