Changes of Biogenic Amines in Chinese Mitten-handed Crab Eriocheir sinensis Stored at Different Temperatures

doi:10.7506/spkx1002-6630-201316068

Abstract

Abstract:

Muscles of Chinese mitten-handed crab, Eriocheir sinensis, were stored at different temperatures, 4, 12 ℃ and
25 ℃, and changes in the content of 8 biogenic amines (BAs) including histamine (His), spermine (Spm), spermidine
(Spd), 2-phenylethylamine (2-Phe), putrescine (Put), cadaverine (Cad), tyramine (Tyr) and tryptamine (Trp) in the
muscle samples were analyzed by UPLC with UV detection. The results indicated that these eight BAs were separated
within 13 min. Linear relationship was obtained within the range of 0.5–20 μg/mL (r＞0.999). The average recoveries
ranged from 84.6% to 98.3%, with RSD less than 10%. Significantly slower changes in the content of the BAs were
observed during storage at a lower temperature (4 ℃). Moreover, Put, Cad, His and Tyr had a significant relation with
TVB-N (P ＜ 0.05).

Key words: Chinese mitten-handed crab, biogenic amines, temperature, UPLC

MENG Yong，ZHANG Yang，HUANG Hong-bing，ZHANG Mei-qin，WU Guang-hong. Changes of Biogenic Amines in Chinese Mitten-handed Crab Eriocheir sinensis Stored at Different Temperatures[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316068.

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Changes of Biogenic Amines in Chinese Mitten-handed Crab Eriocheir sinensis Stored at Different Temperatures

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