Abstract:

The in vitro antioxidant activity of herbacetin was investigated in terms of scavenging activities against DPPH
and hydroxyl free radicals and inhibitory effects against oxidative protein damage and carbonylation induced by Cu2+-
H2O2 or AAPH. At a concentration between 10 μmol/L and 100 μmol/L, herbacetin exerted a marked scavenging effect on
DPPH and hydroxyl free radicals, with respective IC50 values of 49.28 μmol/L and 219.2 μmol/L. Meanwhile, herbacetin could
significantly inhibit oxidative protein damage and carbonylation induced by Cu2+-H2O2 or AAPH in a dose-dependent manner.
This study concludes that herbacetin has a strong ability to scavenge free radical and inhibit oxidative protein damage.

Key words: herbacetin, free radical, protein damage, carbonylation, antioxidant activity