Abstract:

Objective: To explore the effect of cold-pressed grape seed superfine powder (CP-GSSP) on visceral anti-oxidative
function in aging mice. Methods: Sixty mice were randomly divided into CP-GSSP low, medium and high dose groups (323,
646, 1938 mg/(kg•d)), VE group (25 mg/(kg•d)), aging model group and normal control group. The mice in the normal control
group were intraperitoneally injected with physiological saline, whereas, other groups were intraperitoneally injected with
D-galactose to establish aging mouse model. Along with intraperitoneal injection, the mice from the CP-GSSP groups, VE
group, aging model group and normal control group were intragastrically administered with CP-GSSP at various doses, VE,
edible oil and edible oil, respectively. Forty-five days later, the content of MDA, and the activities of SOD and
GSH-Px in heart, kidney and liver were detected. Results: CP-GSSP resisted the aging performance caused by D-galactose
without causing adverse effects in viscera, and significantly reduced the content of MDA, and improved the activities of
SOD and GSH-Px in heart, kidney and liver. Conclusion: CP-GSSP can inhibit lipid peroxidation and improve the activity of
endogenous antioxidases in aging mice. Therefore, it has a good antioxidant and anti-aging effect.

Key words: cold-pressed grape seeds, superfine powder, viscera, antioxidation