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Optimization of Alkaline Extraction and Acid Precipitation for the Extraction of Okra Seed Protein

LI Jia-xing,XIANG Dong,ZHOU Yan-hui,HUANG Cheng   

  1. 1. Institute of Food Science, Jishou University, Jishou 416000, China;
    2. Hunan Amazing Grace Biotechnology Co. Ltd., Changsha 410008, China
  • Online:2013-10-25 Published:2013-09-28

Abstract:

The extraction process for okra seed protein by alkaline extraction and acid precipitation method was optimized.
Four process conditions including solid/solvent ratio, extraction time and temperature and solvent pH were investigated for
their effect on the extraction efficiency of okra seed protein and optimized using an L9(34) orthogonal array design. Results
showed that the optimal extraction conditions were solid/solvent ratio of 1:30 (g/mL), extraction time of 60 min, extraction
temperature of 50 ℃, and extraction pH of 9.0. Under these conditions, the yield of okra seed protein was 72.3%.

Key words: okra, alkaline extraction and acid precipitation, protein, orthogonal array design, process conditions