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Determination of α-Naphthaleneacetic Acid in Vegetables and Fruits by Flow Injection Chemiluminescence with Aqueous Two-Phase Extraction

GAO Xiang-yang，WANG Shan，CHEN Shuang，ZHU Ying-rui   

  1. 1. School of Food Science and Engineering, University for Science and Technology Zhengzhou, Zhengzhou 450064, China；
    2. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China；
    3. Zhengzhou Supervision and Testing Center for Quality and Technology, Zhengzhou 450006, China
  • Online:2013-10-25 Published:2013-09-28

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Abstract:

A flow injection chemiluminescence method was established for the fast determination of α-naphthaleneacetic
acid (NAA) in vegetables and fruits based on its suppressive effect on the reaction between Luminol and hydrogen
peroxide. NAA residue was extracted with ethanol/ammonium sulfate. The linear range of this method was 1.0 × 10-9
to 1.0 × 10-4 mol/L, with correlation coefficient of 0.9976, the detection limit was 3.98 ng/g, the relative standard
deviation (RSD) was 3.2% for 11 parallel determinations, and the recovery rate was 88.99% to 92.1%. This method had the
advantages of wide linear range, high sensitivity and ease of automatic operation and was applied with satisfactory results in
the determination of NAA in vegetables and fruits.

Key words: aqueous two-phase extraction；flow injection-chemiluminescence；vegetables and fruits；&alpha, -naphthaleneacetic acid