Comparative Study of Protein, Lysine and Glutathione Extracted from Wheat Germ or Endosperm in Five Cultivars

doi:10.7506/spkx1002-6630-201321020

Abstract

Abstract:

Purpose: To compare the content and properties of protein, lysine and glutathione extracted from wheat germ or
endosperm from five cultivars (Zhoumai 16, spelt 7, Zhongyou 9507, spelt 53 and Xiaoyan 54). Methods: Protein properties were
characterized by SDS-PAGE and IEF-PAGE. The contents of protein, lysine and glutathione were determined using Folin-phenol
assay, ninhydrin reaction and DTNB colorimetry, respectively. Results: The composition and number of protein subunits of wheat
germ were different from those of endosperm in each cultivar. The molecular weights and isoelectric points of protein subunits had
similar distribution range between wheat germ and endosperm in five cultivars. The contents of wheat germ protein and glutathione
indicated significant differences among different cultivars (P ＜ 0.05 or P ＜ 0.01). Protein, lysine and glutathione contents of
wheat germ from the same cultivar were much higher than those of endosperm with significant difference (P ＜ 0.05 or P ＜ 0.01).
Conclusions: Inter-cultivar diversities of wheat germ protein and glutathione contents existed resulting from germplasm difference,
and the contents of protein, lysine and glutathione in wheat germ were commonly higher than in endosperm. As a whole, Zhongyou
9507 was the best in quality.

Key words: wheat germ, endosperm, protein, lysine, glutathione

CLC Number:

TS209

ZHANG Yan-zhen，GAO Yan，ZHANG Jing，CHEN Wen*. Comparative Study of Protein, Lysine and Glutathione Extracted from Wheat Germ or Endosperm in Five Cultivars[J]. FOOD SCIENCE, 2013, 34(21): 96-102.

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