Optimization of Aqueous Enzymatic Extraction of Hemp Seed Oil and Analysis of Its Fatty Acid Composition

doi:10.7506/spkx1002-6630-201322006

Abstract

Abstract:

The yields of hemp seed oil from peeled hemp seeds by aqueous enzymatic extraction with Protex 6L, Protex 7L,
Alcalase 2.4L, Viscozyme L and Babaowei 2X were 3.62%, 36.88%, 17.05%, 50.98%, and 33.33%, respectively. Viscozyme L
was chosen as the best enzyme according to the oil contents in the emulsions, hydrolysates and precipitates. The enzymatic
hydrolysis process was optimized by response surface methodology to obtain maximum free oil yield. As a result, the optimal
hydrolysis conditions were determined as 7000 U/g of enzyme dosage, 60 ℃, initial pH 5, 1:6 of solid/liquid ratio and 3 h.
By using GC analysis, 11 fatty acids were detected in the seed oil, including saturated fatty acids (mainly palmitic, arachidic
and stearic acid) and unsaturated fatty acids (mainly oleic, linoleic and α-linolenic acid). Among these fatty acids, the most
abundant was linoleic acid with a relative content of 47.5%.

CLC Number:

WANG Huan，LI Yang，JIANG Lian-zhou，WANG Yan，WANG Zhong-jiang，FENG Hong-xia，FENG Dan，LIU Hai-ying. Optimization of Aqueous Enzymatic Extraction of Hemp Seed Oil and Analysis of Its Fatty Acid Composition[J]. FOOD SCIENCE, 2013, 34(22): 27-32.

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