Simultaneous Determination of Jujuboside A, Jujuboside B and Spinosin in Ziziphi Spinosae Semen Dispersible Tablets by HPLC-MS-MS

doi:10.7506/spkx1002-6630-201322030

Abstract

Abstract:

Objective: A method for simultaneous determination of jujuboside A (JuA), jujuboside B (JuB) and spinosin in
ZSS (Ziziphi Spinosae Semen) dispersible tablets was established using high performance liquid chromatography tandem
electrospray ionization mass spectrometry (HPLC-ESI-MS-MS). Methods: The separation was performed on a YMC ODSAQ
TM column with acetonitrile-0.1% formic acid (36:64, V/V) as the mobile phase at flow rate of 0.3 mL/min. The column
temperature was 30 ℃. The injection volume was 20 μL. The analytes were detected by ESI-MS in the negative ion mode.
In the multiple reaction monitoring (MRM) mode, the quantitative determination of JuA at m/z of 1205.6-1073.6, JuB at m/z
of 1043.6-911.7, spinosin at m/z of 607.2-427.0 was carried out. Results: A good linear relationship between the peak area
and the concentrations of JuA, JuB and spinosin was obtained (r ＞ 0.9992). The average recovery rates for them were 97.39%,
97.86% and 98.48%, respectively, with relative standard deviation less than 1.0%. The contents of JuA, JuB and spinosin
in ZSS dispersible tablets were 4.15, 1.46 mg/g and 21.46 mg/g, respectively. The developed method is rapid, accurate and
repeatable, which is suitable for the simultaneous determination of JuA, JuB and spinosin in ZSS dispersible tablets.

Key words: Ziziphi Spinosae Semen (ZSS) dispersible tablet, jujuboside A (JuA), jujuboside B (JuB), spinosin, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS), multiple reaction monitoring (MRM)

CLC Number:

R944.4

MA Gui-jie，ZHANG Ting，XIE Jun-bo，PANG Guang-chang. Simultaneous Determination of Jujuboside A, Jujuboside B and Spinosin in Ziziphi Spinosae Semen Dispersible Tablets by HPLC-MS-MS[J]. FOOD SCIENCE, 2013, 34(22): 150-153.

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