FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (22): 208-211.doi: 10.7506/spkx1002-6630-201322042

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Determination of Estradiol Benzoate Residues in Aquatic Products by LC-MS-MS

ZHANG Shan-shan,YU Hui-juan,HUI Yun-hua,FENG Bing,ZHANG Xiao-ling   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    2. Laboratory of Aquatic Products Quality Safety and Processing, East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Shanghai 200090, China
  • Received:2013-07-01 Revised:2013-10-11 Online:2013-11-25 Published:2013-12-05
  • Contact: Yu Hui-Juan E-mail:yuhuijuan607@sohu.com

Abstract:

A high performance liquid chromatography-tandem mass spectrometric (LC-MS-MS) method has been
established for the determination of estradiol benzoate residues in aquatic products. Sample pretreatment conditions and
instrumental parameters were optimized. Samples were extracted with methyl tert-butyl ether, cleaned up on an LC-C18 solid
phase extraction column and degreased with methanol-water. Separation of the analyte was carried out on Waters XBridge
RP-18 column (150 mm × 4.6 mm i.d., 5 μm) using a mobile phase made up of methanol and water before analysis by
electrospray ionization LC-MS-MS using medroxyprogesterone-D3 as internal standard. The limit of quantification of this
method was 1 μg/kg. The average recovery from samples spiked in the range of 1 to 10 μg/kg was 77.9%–113.5%, with
RSD less than 9.4%. This method was sensitive, reproducible, and suitable for the determination of estradiol benzoate in
various aquatic products.

Key words: estradiol benzoate, aquatic products, enzymatic hydrolysis, high performance liquid chromatographytandem mass spectrometry (LC-MS-MS)

CLC Number: