FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (22): 273-276.doi: 10.7506/spkx1002-6630-201322055

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Separation and Purification of Small-Molecule Peptides with Immunomodulating Activity from Goat Placenta

LIU Long-xing,REN Xing-hong,TANG He-jing,TANG Zhao-yong,HE Cheng-ming,FANG Liao-qiong   

  1. 1. College of Biotechnology, Southwest University, Chongqing 400715, China;
    2. School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
  • Received:2012-11-25 Revised:2013-10-13 Online:2013-11-25 Published:2013-12-05

Abstract:

The purpose of this study was to isolate and purify small-molecule peptides with immunomodulating activity
from goat placenta. Ultrafiltration fraction from goat placenta was prepared by a conventional method, followed by
isolation, purification and analysis by reversed phase-high performance liquid chromatography (RT-HPLC). The immunomodulating
activity of its components was measured by E-rosette test and the N-terminal sequence was measured
by Edman degradation reaction. Three small-molecule peptides with high purity were obtained, two of which were immune-
active. The results of sequencing and retrieving showed that they were new peptides. RT-HPLC was one of the
optimal methods to purify and analyze bioactive peptides. The small-molecule peptides from goat placenta revealed
strong immune activity.

Key words: goat placenta small-molecule peptides, reversed phase-high performance liquid chromatography (RT-HPLC), E-rosette

CLC Number: