Isolation, Purification and Compositional Analysis of Agaropectin from Gracilaria lemaneiformis

doi:10.7506/spkx1002-6630-201323034

Abstract

Abstract:

Three polysaccharides named as SPS-1, SPS-2 and SPS-3 were separated by gradient elution on a DEAEcellulose
column from the agaropectin extracted from Gracilaria lemaneiformis. Their chemical analysis including sulfate
base content, relative molecular mass, purity, characteristic bonds and monosaccharide composition were characterized byion
chromatography, high performance liquid chromatography, ultraviolet spectroscopy and infrared spectroscopy. The results indicated that the
yields of SPS-1, SPS-2 and SPS-3 were 2.7%, 47.2% and 2.2%, with sulfate base contents of 5.1%, 9.3% and 6.3%, respectively.
The agaropectin contained no protein, amino acid or nucleic acid. The content of SPS-2 consisting of polysaccharides of similar chain
length of was 92.14%. The mass-average and number-average relative molecular masses of SPS-2 were 367686 and 230434,
respectively. SPS-2 was a group of complex sulfated polysaccharide with 17.34% galactose, 2.72% raffinose, 8.82% saccharose
and 1.18% arabinose in the monosaccharide composition.

Key words: agaropectin, isolation and purification, IR analysis, monosaccharide composition

CLC Number:

Q819

LIU Qiu-feng，SU Yong-chang，WU Cheng-ye*. Isolation, Purification and Compositional Analysis of Agaropectin from Gracilaria lemaneiformis[J]. FOOD SCIENCE, 2013, 34(23): 160-164.

References

[ ] 国家医药管理局中草药情报中心站.植物药有效成分手册[M].北京, 人民卫生出版社, 1986,287.
[ ] 王新梅,闰美艳.海藻多糖生物活性研究进展[J].齐鲁药事,2009,28(4):228-231.
[ ] 宫春宇,唐庆九,张劲松,等.龙须菜免疫活性多糖的分离纯化及结构鉴定[J].天然产物研究与开发,2010,22:603-606,624.
[ ] Marinho-Soriano E..Agar polysaccharides from Gracilaria species(Rhodophyta,Gracilariaceae)[J]. Biotechnology, 2001, 89:81-84.
[ ] 卢佳芳,杨文鸽,颜伟华.龙须菜(Gracilaria lemaneiformis)多糖的分离纯化及其组成研究[J].海洋与湖沼,2009,40(4):484-488.
[6] 赵谋明,邱慧霞,彭志英.江蓠琼胶提胶新工艺的研究[J].食品与发酵工业,1997,23(2):3-7.
[7] 刘秋凤,苏永昌,吴成业.硫琼脂提取工艺的响应面法优化[J].福建农业学报,2012,27(9):1004-1010.
[ ] 穆凯峰,吴永沛.坛子菜多糖的分离纯化及其组成分析[J].中国食品报,2011,11(1):159-164.
[ ] 国家药典委员会. YBZ00902003-2008褐藻多糖硫酸酯[S].国家食品药品监督管理局,2008.
[ ] 法国核岛部件设计建造规则协会（AFCEN）.RCC-M - Edition 2007 SECTION III Examination Methods(MC)[S].法国 ,2007.
[ ] 杨贤庆,赵永强,戚勃,等.离子色谱法与硫酸钡比住房测定琼胶糖中硫酸基含量的比较研究[J].食品科学,2009,30(18):340-343.
[ ] 张文清,左萍萍, 徐辰,等. 海带中岩藻多糖的分离纯化与结构分析[J].食品科学,2012,33(1):68-71.
[ ] 中国国家标准化管理委员会. GB/T 6040-2002.红外光谱分析方法通则[S].北京:中国标准出版社,2008.
[ ] 李晋,徐怀德,米林峰. 洋葱多糖的分离纯化及单糖组成研究[J].中国食品学报, 2012, 12(2):202-206.
[ ] 刘力.琼脂分级产物的研究[D].青岛:中国科学院海洋研究所,2001.
[ ] Duckworth M.,Hong K.C. and Yaphe W..The agar polysaccharides of Gracilaria species Carbohyd[J]. Carbohydrate Research,1971,1-9.
[17] Araki C..Structhre of the agarose consituent of agar-agar[J].Bull. Chem. Soc. Jap.,1956,29:543-544.
[18] Clingman A.L.,Nunn J.R. and Stephen A.M..Red-seaweed polysaccharides.Part I. Gracilaria confervoides[J].J. Chem. Soc.,1957:197-203.
[19]Friedlander M.,Lipkin Y. and Yaphe W..Composition of agars from Gracilaria cf. verrocosa and Pterocladia capillacea[J].Bot. Mar.,1981,26:595-598.
[20] Izumi K..Chemical heterogeneity of the agar from Gracilaria verrocosa[J].J. Biochem.,1972,72:135-140.

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