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Determination of Octylphenol, Nonylphenol and Bisphenol A in Aquatic Products by Gel Permeation Chromatography Coupled with Solid Phase Extraction and Gas Chromatography-Mass Spectrometry

HUANG Hui，DENG Xu-xiu，ZHANG Hua-wei，LIU Yun，XU Ying-jiang，GONG Xiang-hong，ZHANG Xiu-zhen   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China；2. Marine Fisheries Research Institute of Shandong Province, Yantai 264006, China；3. Shandong Fishery Environmental Monitoring Station, Yantai 264006,China；4. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China
  • Online:2013-12-25 Published:2014-01-03

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Abstract:

On the basis of optimized sample pretreatment conditions, a method for the determination of octylphenol (OP),
nonylphenol (NP) and bisphenol A (BPA) in aquatic products has been developed by gel permeation chromatography
(GPC) coupled with solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). The analytes were
extracted with ethyl acetate and purified seuquentially by gel permeation chromatography and HLB solid phase extraction
cartridges. After derivatization with heptafluorobutyrican anhydride (HFBA), the derivatives were determined by GCMS.
The results showed that the derivatives of the analytes were well separated by an HP-5MS column, and the limits of
detection for OP, NP and BPA were 0.2, 0.1 μg/kg and 0.1 μg/kg, respectively. The average recovery rates were more than
83.4%, 80.5% and 81.3%, respectively, with relative standard deviation (RSD) ranging from 3.73% to 9.19%. The method
has been used to determine seafood samples from Laizhou Bay with high sensitivity and reliability.

Key words: octylphenol, nonylphenol, bisphenol A, gas chromatography-mass spectrometry (GC-MS), gel permeation chromatography (GPC), solid phase extraction (SPE), aquatic product