Factors Influencing the Dissociation of Duck Breast Muscle Actomyosin

doi:10.7506/spkx1002-6630-201401005

Abstract

Abstract:

This study illustrated multiple potential factors influencing actomyosin dissociation, including internal factors(AMP, IMP, ADP, ATP, Ca2+, and PO4
3-) and external factors (marinated with NaCl or with different phosphate salts).Actomyosin was extracted from duck breast muscle and the protein composition was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western-blot analysis. Results: The density of actin bands was increased significantly when the actomyosin was treated with 8, 16 mmol/L AMP or IMP or with 25–50 mmol/L PO4 3- at 4 ℃, whereas treatment with 8, 16 mmol/L ADP or with 0.1–5 mmol/L Ca2+ did not result in a significant change in actin band densities.These results suggest that marinating duck muscle with different salts has no significant effects on actomyosin dissociation.

Key words: duck, actomyosin, dissociation, affecting factors

CLC Number:

TS251.68

DONG Han1,2, WANG Dao-ying1, ZHANG Mu-han1, ZHU Yong-zhi1, XU Wei-min1,*, LIU Fang1. Factors Influencing the Dissociation of Duck Breast Muscle Actomyosin[J]. FOOD SCIENCE, 2014, 35(1): 23-28.

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