Combinatorial Induction of Recombinant Dextransucrase Expression in E. coli by IPTG and Lactose

doi:10.7506/spkx1002-6630-201401036

Abstract

Abstract:

In order to explore the combinatorial induction of recombinant dextransurase experssion in E. coli by isopropyl
β-D-1-thiogalactopyranoside (IPTG) and lactose, a mixture of IPTG and lactose was added to the cell culture to induce the
expression of recombinant dextransurase. Based on the optimal culture medium, IPTG concentration and the amount of
lactose added, bacteria concentration and induction time revealed significant impacts on the enzyme activity. The results
showed that the optimal concentrations of IPTG and lactose were 0.1 mmol/L and 2.5 g/L, respectively, and optimal
induction time was 4 h at 25 ℃. Under these culture conditions, the activity of dextransucrase could reach up to 40.44 U/mL. The
combinatorial induction of recombinant E. coli dextransurase by IPTG and lactose was feasible, greatly reducing the dosage
of IPTG and industrial production costs.

Key words: isopropyl-β-D-1-thiogalactopyranoside (IPTG), lactose, dextransucrase, expression, combinatorial induction

CLC Number:

Q814

LI Pan-feng, ZHANG Hong-bin*, HU Xue-qin. Combinatorial Induction of Recombinant Dextransucrase Expression in E. coli by IPTG and Lactose[J]. FOOD SCIENCE, 2014, 35(1): 185-188.

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