Rice Bran Sterol Ameliorates Non-alcoholic Steatohepatitis in Rats

doi:10.7506/spkx1002-6630-201401044

Abstract

Abstract:

Aim: To investigate the effect of rice bran sterol (RBS) on non-alcoholic steatohepatitis (NASH) in Sprague-Dawley
(SD) rats caused by feeding high-fat diet for 13 weeks. Methods: Totally 50 male SD rats were randomly assigned to control group,
NASH group and three RBS treatment groups at doses of 65, 130 mg/kg and 195 mg/kg. The NASH group and RBS treatment
groups were fed with high-fat diet and the RBS groups were administered with RBS by gavage at the same time. Two percent
Tween-80 was added to improve the solubility of RBS. After feeding for 13 weeks, serum total cholesterol (TC), triglyceride
(TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and the activities of serum
glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), nitric oxide synthetase (NOS) and superoxide
dismutase (SOD) were measured, and organ indexes were compared among groups. Results: Severe steatosis in the NASH group
was observed through examining pathological slice of the liver. Serum HDL-C, SOD, NOS, ALT and AST levels in the NASH
group significantly abnormal when compared with those in the control group. Serum HDL-C level of the RBS middle-dose group
was significantly increased (P ＜ 0.05) and serum ALT levels of all RBS treatment groups were decreased (P ＜ 0.001), while AST/ALT
ratio was increased when compared with the NASH group (P ＜ 0.01). Serum SOD and NOS activities in the low-dose group
were significantly decreased when compared with those in the NASH group. Conclusion: Moderate dosage of rice bran sterol can
ameliorate NASH in rats caused by long-term high-fat diet feeding.

Key words: rice bran sterol, non-alcoholic steatohepatitis, serum lipids, antioxidation, glutamic-oxalacetic transaminease/ glutamicpyruvic transaminase ratio, liver tissue slice

CLC Number:

R151.3

WANG Yi-ran1,2, HE Lan3, LIU Jin-hui4, XU Fang1,2, LIU Xiang-xin4, HE Jian-hua2,*. Rice Bran Sterol Ameliorates Non-alcoholic Steatohepatitis in Rats[J]. FOOD SCIENCE, 2014, 35(1): 224-228.

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