Determination of Abietic Acid in Duck Skin Tissue by Solid Phase Extraction-High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201404017

Abstract

Abstract:

A high-performance liquid chromatographic (HPLC) method was established to determine abietic acid in duck
skin. Abietic acid in duck skin samples was extracted with acetonitrile, followed by purification with a C18 solid-phase
extraction cartridge, and determined by HPLC with an ultraviolet (UV) detector. The chromatographic conditions were
established using a C18 column (250 mm× 4.6 mm, 5 μm) with a mobile phase consisting of 0.003 mol/L phosphoric acidmethanol
(8:92, V/V) at a flow rate of 1.0 mL/min and the detection wavelength was set at 240 nm. Results indicated that
the linearity ranged from 0.1 to 10.0 mg/L, the detection limit of abietic acid was 0.1 μg/g, and the quantification limit was 0.33 μg/g.
The recoveries from spiked samples at concentration levels of 0.5–50 μg/g were in the range of 82.2%–88.0%. The
developed method was successfully applied to the determination of abietic acid in duck samples epilated with colophony.
The content of abietic acid in the duck sample could reach 14.52 μg/g. This method proved to be of high maneuverability as
well as excellent sensitivity and accuracy, and could be used for quantitative analysis of abietic acid in duck skin tissue.

Key words: duck, rosin, abietic acid, high performance liquid chromatography (HPLC), solid phase extraction (SPE)

CLC Number:

TS207
O657

ZHANG Su-zhen1,2, GENG Zhi-ming1, WANG Dao-ying1, ZHU Yong-zhi1, LIU Fang1, ZHANG Mu-han1, BIAN Huan1, XU Wei-min1,*. Determination of Abietic Acid in Duck Skin Tissue by Solid Phase Extraction-High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2014, 35(4): 82-85.

References

[1] Ozaki A, Ooshima T, Mori Y. Migration of dehydroabietic and abietic acids from paper and paperboard food packaging into food-simulating solvents and Tenax TA[J]. Food additives and contaminants. 2006, 23(8): 854-860.

[2] Tavendale M H, Mcfarlane P N, Mackie K L, et al. The fate of resin acids-1. The biotransformation and degradation of deuterium labelled dehydroabietic acid in anaerobic sediments[J]. Chemosphere. 1997, 35(10): 2137-2151.

[3] Nestmann E R, Lee E G, Matula T I, et al. Mutagenicity of constituents identified in pulp and paper mill effluents using the Salmonella/mammalian-microsome assay[J]. Mutation Research/Genetic Toxicology. 1980, 79(3): 203-212.

[4] Sunzel B, S?derberg T A, Reuterving C, et al. Neutralizing effect of zinc oxide on dehydroabietic acid-induced toxicity on human polymorphonuclear leukocytes[J]. Biological trace element research. 1991, 30(3): 257-266.

[5] S?derberg T A, Johansson A, Gref R. Toxic effects of some conifer resin acids and tea tree oil on human epithelial and fibroblast cells[J]. Toxicology. 1996, 107(2): 99-109.

[6] Ozaki A, Yamaguchi Y, Fujita T, et al. Safety assessment of paper and board food packaging: Chemical analysis and genotoxicity of possible contaminants in packaging[J]. Food additives and contaminants. 2005, 22(10): 1053-1060.

[7] Kamaya Y, Tokita N, Suzuki K. Effects of dehydroabietic acid and abietic acid on survival, reproduction, and growth of the crustacean Daphnia magna[J]. Ecotoxicology and environmental safety. 2005, 61(1): 83-88.

[8] Lee B L, Koh D, Ong H Y, et al. High-performance liquid chromatographic determination of dehydroabietic and abietic acids in traditional Chinese medications[J]. Journal of Chromatography A. 1997, 763(1): 221-226.

[9] Mitani K, Fujioka M, Uchida A, et al. Analysis of abietic acid and dehydroabietic acid in food samples by in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry.[J]. Journal of chromatography. A. 2007, 1146(1): 61.

[10] Nilsson U, Berglund N, Lindahl F, et al. SPE and HPLC/UV of resin acids in colophonium‐containing products[J]. Journal of separation science. 2008, 31(15): 2784-2790.

[11] Xiao C, Rao W W, Zhong M C. [Study on determination methods for abietic acid in fake Myrrha].[J]. Zhong yao cai= Zhongyaocai= Journal of Chinese medicinal materials. 2012, 35(8): 1237-1241.

[12] Hroboňová K, Lehotay J, Ska?áni I, et al. HPLC determination and MS identification of dehydroabietic acid and abietic acid in propolis[J]. Journal of liquid chromatography & related technologies. 2005, 28(11): 1725-1735.

[13] Chow S Z, Shepard D. High performance liquid chromatographic determination of resin acids in pulp mill effluent[J]. Tappi journal. 1996, 79(10): 173-179.

[14] Lee B L, Ong H Y, Koh D, et al. High-performance liquid Chromatographie method for determination of dehydroabietic and abietic acids, the skin sensitizers in bindi adhesive[J]. Journal of Chromatography A. 1994, 685(2): 263-269.

[15] 仇雅静. 高效液相色谱法检查骨筋丸胶囊中非法添加的松香酸[J]. 中国药业. 2011, 20(004): 39-40.

[16] 李富贤, 米彩峰, 石会丽. RP—HPLC 测定清毒素胶囊中松香酸的含量[J]. 中国中药杂志. 2008, 33(9): 1086-1087.