Abstract:

In this paper, immune adsorbents were prepared for the adsorption of aflatoxin M1 in milk. Copolymer
microspheres of glyceryl methacrylate (GMA) and methyl methacrylate (MMA) were prepared using dispersion
polymerization with azodiisobutyronitrile (AIBN) as initiator in aqueous ethanol medium in the presence of
polyvinylpyrrolidone (PVP) as dispersant under various conditions. Anti-AFM1 monoclonal antibody was coupled with the
epoxy groups on the microsphere surface to form an immune adsorbent. The microsphere particle size was approximately
1.7 μm, and the quantity of epoxy group in the microspheres was between 67.2% and 71.6%. The adsorption quantity of
monoclonal antibodies for aflatoxin M1 in milk was more than 1.2 μg/g. These results show that the selective adsorption
for aflatoxin M1 in milk of the immune adsorbent is good. It can be an ideal immune adsorption material for the removal of
aflatoxin M1 in milk.

Key words: copolymer microspheres, aflatoxin M1, immune adsorbent, adsorption