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Separation and Purification of Free Radical Scavenging Bioactive Components from Endophytic Fungi in Ligustrum lucidum

CHEN An-hui, CHEN Hong-wei, SHAO Ying, ZHANG Di   

  1. College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Online:2014-05-15 Published:2014-05-19

Abstract:

Endophytic fungi were isolated from Ligustrum lucidum by tissue separation and their 1,1-diphenyl-2-
picrylhydrazyl (DPPH) radical scavenging activity was studied. Metabolite components with high DPPH radical scavenging
activity were purified. One hundred and one strains of endophytic fungi were isolated from leaves, stems, roots and branches.
It was found that NZ-18 strain had the highest DPPH radical scavenging activity and an active component was prepared from
its fermentation metabolites by liquid chromatography, which was identified as a pure compound by HPLC and TLC and named
as compound 1. The compound I at a concentration of 0.5 mg/mL could scavenge 94.88% of DPPH at 0.2 mg/mL. The present study
indicated that there were many endophytic strains from Ligustrum lucidum whose metabolites could scavenge DPPH free radical.

Key words: Ligustrum lucidum, endophytic fungi, free radical, component, purification