Isolation and Identification Based on Type I PKS Functional Gene of Endophyte from Salicornia bigelovii

doi:10.7506/spkx1002-6630-201411023

Abstract

Abstract:

In this study, a fungal endophyte with type Ⅰ polyketide synthase (PKS) named as Salix 1 was isolated from
wild Salicornia bigelovii collected from a salt lake in Xinjiang by streak plate and slant culture methods. The 18S rDNA and
ITS1-5.8S-ITS2 rDNA sequences from the strain were amplified by PCR and sequenced for sequence homology analysis
using NCBI BLAST, and phylogenetic trees were constructed. Based on phylogenetic analysis combined with morphological
observation, the strain Salix 1 was identified as Aspergillus versicolor.

Key words: Salicornia bigelovii, endophyte, type Ⅰ PKS gene, isolation and identification

LIU Yi-jun, PAN Mu, WANG Ming-yang, WANG Hui, XIN Zhi-hong. Isolation and Identification Based on Type I PKS Functional Gene of Endophyte from Salicornia bigelovii[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201411023.

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Isolation and Identification Based on Type I PKS Functional Gene of Endophyte from Salicornia bigelovii

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