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Isolation, Identification, Degradation Characteristics of a Bacterial Strain Capable of Degrading Cypermethrin

TANG Jie, ZENG Chao-yi, ZHANG Qing, SHI Ying   

  1. School of Bioengineering, Xihua University, Chengdu 610039, China
  • Online:2014-06-15 Published:2014-07-03

Abstract:

A bacterial strain named N-02 capable of degrading cypermethrin was isolated from long-term cypermethrin
contaminated orchard soil by using enrichment culture. Based on the morphology, physio-biochemical characteristics, and
16S rDNA sequence analysis, strain N-02 was identified as Aeromonas punctata. The degradation rate of cypermethrin was
62.31% when the strain were cultured at 35 ℃ for 96 h in LB medium containing 20 mg/L cypermethrin at pH 7.5. A wide
degradation spectrum of pyrethroid by strain N-02 was observed, and the degradation rates of β-cypermethrin, deltamethrin
and fluoride cypermethrin after 96 h were 40.17%, 33.26% and 30.45%, respectively.

Key words: cypermethrin, Aeromonas punctata, degradation characteristics, degradation spectrum