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Antioxidant Activities in vitro and in Cells and Amino Acid Composition of Rapeseed Protein Hydrolysates (RPHs)

WANG Li-feng, WANG Yu-mei, ZHANG Jing, XIE Hui-hui, JU Xing-rong   

  1. School of Food Science and Engineering, Nanjing University of Finances and Economics, Nanjing 210023, China
  • Online:2014-07-15 Published:2014-07-18

Abstract:

In this study, rapeseed proteins obtained from defatted meal of double-zero rapeseed (Qinyou No. 7) by alkaline
extraction and acid precipitation were hydrolyzed with Alcalase 2.4L to produce rapeseed protein hydrolysates (RPHs). After
chromatographic separation of RPHs on a Sephacryl S-100HR column, three fractions were harvested. Fraction III showed
the most potent antioxidant activity in terms of oxygen radical absortion capacity (ORAC) and cellular antioxidant capacity
(CAA). This fraction had an ORAC value of (1 610.38 ± 112.51) μmol TE/g, a CAA value of (124.66 ± 2.18) μmol QE/g,
and an EC50 value of (57.84 ± 3.38) μg/mL. Furthermore, the results of amino acid composition analysis and electrophoresis
indicated a possible association between the antioxidant activities and either molecular weight distribution or amino acid
composition.

Key words: rapeseed protein hydrolysates, oxygen radical absortion capacity (ORAC), cellular antioxidant capacity (CAA), amino acid analysis, electrophoresis