Abstract:

Objective: The effect of casein glycomacropeptide (CGMP) on cell proliferation and on the expression of
cyclooxyenase-2 (COX-2), inducible nitrite oxide synthase (iNOS), and glutathione-S-transferase π (GST-π) in HT-29
cells was explored to provide a reliable basis for the use of CGMP as an ingredient in functional foods. Methods: HT-29
Cells were cultured with CGMP for 12, 24 and 48 h. The inhibition of HT-29 cells proliferation was measured by MTT
[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The expression of COX-2, iNOS, and GST-π was
detected by reverse transcriptionpolymerase chain reaction (RT-PCR) after the HT-29 cells were treated for 24 h. Results: 1)
CGMP inhibited the proliferation of HT-29 cells in a time-dependent fashion, and the optimal concentration was 10-4 mg/mL.
2) Low doses of CGMP (10-5, 10-4 and 10-3 mg/mL) significantly reduced the expression of three genes. Conclusion: To some
extent, CGMP can inhibit the proliferation of HT-29 cells in a time-dependent manner. The mechanism is associated with
decreasing the expression of COX-2, iNOS and GST-π by CGMP and further improving human colorectal cancer.

Key words: casein glycomacropeptide (CGMP), human colonic tumor cells HT-29, COX-2, iNOS, GST-π