Abstract:

Macroporous resins D101-1and AB-8 were found more suitable to purify flavonoids from Zostera marina L., as
indicated by comparing their dynamic adsorption and desorption capacities with those of three other kinds of macroporous
resin. The purification process was optimized by combined use of the two selected resins. The optimal mixing ratio of D101-1
to AB-8 was 2:3. The best purification results were achieved by loading 6 BV (bed volume) of the sample containing 1.25 mg/mL
flavonoids at pH onto the mixed column at a flow rate of 3 BV/h and subsequently eluting the column with 5 BV of 70%
ethanol at a flow rate of 3 BV/h. After the purification, the total flavonoid content was increased from (12.66 ± 0.42)% to
(51.25 ± 1.26)%.

Key words: Zostera marina L., flavonoids, macroporous resin adsorption, purification