Abstract:

The purification of total flavonoids extracted from the persistent calyx of Physalis alkekengi L. var. franchetii
(Mast.) Makino using D-101 macroporous resin was optimized by an orthogonal array design involving five independent
variables at four different levels using adsorption and desorption rates of total flavonoids as response variables. The purity of
total flavonoids was increased from 11.45% to 45.56% when the total flavonoid extract from the persistent calyx of Physalis
alkekengi L. var. franchetii (Mast.) Makino at pH 10 was adsorbed by the resin for 3 h and then desorbed with a 35-fold
volume of 80% ethanol for 7 h.

Key words: Physalis alkekengi L. var. franchetii (Mast.) Makino, total flavonoid, macroporous resin, purification