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Proteomic Analysis of Cherry Tomato Fruits under Modified Atmosphere Conditions

ZHOU Xiang, JIANG Li, HUAN Chen, AN Xiu-juan, YU Zhi-fang*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095
  • Online:2014-07-25 Published:2014-08-26
  • Contact: YU Zhi-fang

Abstract:

Proteomic analysis was used to understand the response mechanism of cherry tomato fruits during storage
under modified atmosphere (MA) conditions in this experiment. After two-dimensional electrophoresis and matrix-assisted
laser desorption-ionization time of flight mass spectrometry (MALDI-TOF-TOF-MS), the results showed that there were
39 differently expressed spots (P < 0.05), including 6 functional proteins, stress response and defense (30.77%), energy
(25.64%), cell fate (15.38%), ripening and senescence (5.13%), detoxification (5.13%) and others (17.95%). Among these
proteins, glutathione S-transferase (GST), NADP-malic enzyme, eukaryotic translation initiation factor 5A-4 (eIF5A-4) and
alcohol dehydrogenase 2(ADH 2) were increased by MA treatment, while salicylic acid-binding protein 2 (SABP 2) and
1-aminocyclopropane-1-carboxylate oxidases (ACOs) were suppressed. This study indicated that the MA treatment of postharvest
cherry tomato fruits could induce the stress response and suppress the ethylene metabolism to delay the ripening of
cherry tomato fruits.

Key words: cherry tomato fruit, modified atmosphere, proteomics, delaying ripening and senescence

CLC Number: