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Kinetics of Laccase Production by Batch Fermentation of Endophytic Fungus Phomopsis liquidambari B3

ZHOU Jun, MEI Yan-zhen, YANG Teng, KANG Li, DAI Zhi-dong, DAI Chuan-chao   

  1. 1. Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for
    Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China;
    2. Shangde Biotechnology Company, Danyang 212300, China
  • Online:2014-08-15 Published:2014-08-25

Abstract:

The aim of this study was to establish a kinetic model of laccase production by batch fermentation of the
endophytic fungus Phomopsis liquidambari B3. The experimental results showed that the production of laccase was partially
associated with the growth of P. liquidambari B3. The experimental data were nonlinearly fitted to the Logistic equation
describing cell growth, the Luedeking-Piret equation indicating laccase production and the Luedeking-Piret-Like equation
revealing substrate consumption using MATLAB software, and the optimal parameters and the fermentation kinetic model
were established. Validation experiments revealed the errors between the calculated and experimental values were less than
10%, indicating that the kinetic model can describe the actual fermentation process of P. liquidambari B3.

Key words: endophytic fungus, Phomopsis liquidambari B3, laccase, batch fermentation, kinetic model

CLC Number: