Abstract:

Ten psychrotrophic strains were isolated from refrigerated rabbit meat and identified with 16S rDNA sequencing.
B25 was identified as Serratia, and nine other strains were identified as Pseudomonas. The amount of biogenic amines
produced by these 10 strains was detected by thin-layer chromatography. B25 strain could produce a variety of biogenic
amines, while the other nine strains could not produce any biogenic amine. B25 was inoculated into Pseudomonas broth
with amino acid precursors and incubated for 24 hours. The biogenic amines produced by B25 strain were determined by
high performance liquid chromatography (HPLC). The results demonstrated that the strain had strong ability to produce
putrescine, phenylethylamine and cadaverine which were determined to be 0.41 mg/mL, 0.05 mg/mL and 0.23 mg/mL,
respectively. Therefore, the strain poses a potential threat to the safety of refrigerated rabbit meat products.

Key words: Pseudomonas, biogenic amines, putrescine, thin layer chromatography (TLC), high performance liquid chromatography (HPLC)