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Optimization of Culture Conditions for the Production of Polysaccharides by Athelia rolfsii in a Medium Consisting of Corn Steep Liquor and Starch

MIAO Xiu-yan, LI Hong-mei, WEI Ming, ZHAO Hui, MIN Wei-hong, MA Yan-qiu   

  1. 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;
    2. Library of Jilin Agricultural University, Changchun 130118, China
  • Online:2014-10-15 Published:2014-10-17

Abstract:

This study was intended to employ response surface methodology to establish the optimum culture conditions
and medium components for the production of polysaccharides by Athelia rolfsii AY6657741 isolated from Clivia miniata
Regel. When Athelia rolfsii AY6657741 was inoculated at an inoculum size of 7% by volume to a culture medium containing
35 g/L corn starch, 50 mL/L corn steep liquor, 3.1 g/L NaNO3, 1.0 g/L KH2PO4, 0.5 g/L KCl, 0.25 g/L MgSO4·7H2O,
and 1.4 g/L citric acid (pH 4.55) and cultivated at 29 ℃ with a rotation speed of 200 r/min for 5.5 d, 16.135 g/L crude
polysaccharides were produced.

Key words: Athelia rolfsii, corn steep liquor, corn starch, optimal culture conditions, crude polysaccharides

CLC Number: