Abstract:

Objective: To investigate the preparation and antibacterial effect of chlorogenic acid (CA) nanoliposome.
Methods: CA nanoliposomes were prepared by film-ultrasonic dispersion method and the products were characterized with
scanning electron microscopy (SEM) and particle size analyzer. The effects of cholesterol/lecithin mass ratio, CA/film
(lecithin + cholesterol) mass ratio and ultrasonication time on the encapsulation efficiency of liposomes were investigated.
Finally, the stability and the bacteriostasis ability of the prepared liposomes were evaluated. Results: Oval-shaped liposomes
were prepared by ultrasonication for 15 min at a cholesterol/lecithin mass ratio of 1:8 and a CA/film ratio of 1:10, which
showed regular morphology, a particle size of approximately 110 nm in diameter, good dispersity, an encapsulation
efficiency of up to 87.5%, and a drug loading of up to 36%. UV spectroscopy showed that CA was encapsulated successfully
into nanoliposomes. In vitro test showed that the drug could be sustained-released from liposomes without a burst release
and with stability for 24 h and 14 days. Comparing with CA and tetracycline, the nanoliposomes had better bacteriostasis
ability. Conclusion: CA nanoliposomes prepared by film-ultrasonic dispersion method have a regular shape, good dispersion
ability and persistent antibacterial ability.

Key words: chlorogenic acid, nanoliposomes, preparation, antibacterial activity