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Determination of Four Fat-Soluble Vitamins in Vitamin Tablets by Pressurized Capillary Electrochromatography with UV Detection

ZHENG Shu1, SHI Wen-jun1, WANG Xiao-xi1, LI Jing2, GU Xue1, YAN Chao1,*   

  1. 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China;
    2. Unimicro (Shanghai) Technologies Co. Ltd., Shanghai 201203, China
  • Online:2014-10-25 Published:2014-11-07

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Abstract:

An effective, convenient and fast method for the analysis of fat-soluble vitamins in vitamin tablets was developed
using pressurized capillary electrochromatography (pCEC) with ultra violet (UV) detection. The separations was performed
on a reversed-phase C18 capillary column with a certain detection wavelength-time program. The mobile phase was 95%
(V/V) methanol containing 0.05% TFA (pH 3.3) at a flow rate of 0.2 mL/min with a separation voltage of 15 kV. Four fatsoluble
vitamins (vitamin A, vitamin D3, vitamin E, and vitamin K3) were baseline separated within 5 min in an isocratic
pCEC mode. The detection limits for vitamin A, vitamin D3, vitamin E, and vitamin K3 (RSN = 3) were 1.5, 3.0, 15 and
1.5 μg/mL with good linearity, respectively. The interference on the analysis of vitamin tablets was effectively eliminated.
The recoveries of spiked fat-soluble vitamin samples were 90.0%–110.0% with RSD ranging from 0.53% to 5.46%. This
method is simple, rapid, repeatable and accurate.

Key words: pressurized capillary electrochromatography (pCEC), ultra-violet (UV) detection, fat-soluble vitamin, vitamin tablets

CLC Number: