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Effect of Selenium-Enriched Green Tea on Activity and Expression of Sarco(endo)Plasmic Reticulum Ca2+-ATPase (SERCA) in Rats with Nonalcoholic Fatty Liver Disease (NAFLD)

WANG Feng-jie, CHEN Xian-bing, ZHANG Shu-yu, TAN Zhi-xin, XIANG Guo-min, LIU Jin-hong   

  1. 1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China;
    2. Science and Technology College of Hubei Minzu University, Enshi 445000, China
  • Online:2014-11-15 Published:2014-11-06

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Abstract:

Objective: To investigate the effect of selenium-enriched green tea on the activity and expression of sarco(endo)
plasmic reticulum Ca2+-ATPase (SERCA) on model rats with experimental non-alcoholic fatty liver disease (NAFLD) and
explore the possible mechanisms for the prevention and treatment of NAFLD. Methods: Totally 32 Wistar rats were divided
into 4 groups randomly and fed with standard diet and water (NC), high-fat diet and water (HC), high-fat diet and tea (TC),
high-fat diet and selenium-enriched tea (SeC), respectively. After 9 weeks, the rats were killed to investigate the changes in
Na+-K+-ATPase and Ca2+-ATPase activities. Meanwhile, hepatic tumor necrosis factor-α (TNF-α) and Ca2+ levels and the
protein expression of SERCA in hepatocytes was evaluated by immunohistochemistry (IHC) and Western blotting. Results:
The protein expression of SERCA and the activities of Na+-K+-ATPase and Ca2+-ATPase were significantly decreased and
the Ca2+ level was remarkably increased in HC group. The activity and expression of Na+-K+-ATPase and Ca2+-ATPase in
liver were markedly upregulated in intervention group. On the contrary, the level of Ca2+ in SeC group was greatly reduced
when compared with ordinary tea. Conclusion: Selenium-enriched green tea can improve hepatic energy metabolism
disorder, protect liver function, and prevent liver damage in NAFLD rats.

Key words: selenium-enriched green tea, nonalcoholic fatty liver disease (NAFLD), sarco(endo)plasmic reticulum

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