Abstract:

The conditions for static and dynamic adsorption and desorption by S-8 macroporous resin for enriching laccaic
acid were investigated by measuring the laccaic acid concentration in crude extract and eluates. The results showed that
adsorption capability of S-8 macroporous resin was favorable to laccaic acid. In the static adsorption process, the resin
exhibited maximum saturated adsorption at 30 ℃ after 4.5 hours. When the flow rate of the sample solution was 2 mL/min
the dynamic adsorption achieved the maximum saturation level. The best enrichment results (more than 90% desorption rate)
were obtained when the adsorbed laccaic acid was desorbed using 100 mL of 95% aqueous ethanol with 1.0 mL of 2 mol/L
hydrochloric acid at a flow rate of 3 mL/min. The desorption rate remained as high as 89.50% after twentieth repeated
use, suggesting good reusability of the sorbent. After the enrichment process, the purity of laccaic acid was increased
from 24.77% to 62.92% with a purification factor of 2.54, and the yield of laccaic acid from lac was 0.52%. This study
demonstrates the applicability of S-8 macroporous resin for enriching laccaic acid.

Key words: laccaic acid, macroporous adsorbent resin, enrichment