Abstract:

Commercial fruit juice beverages and their samples inoculated with Alicyclobacillus sp. isolates were
discriminated by an α-Astree Ⅱ electronic tongue with principal component analysis. Except for two beverages mainly
containing dairy components, other samples could be separated in different groups. Six beverages that could be discriminated
by E-tongue were inoculated with two Alicyclobacillus sp. isolates and were incubated at 45 ℃ for 30 days. All inoculated
samples were detected with colonies on Bacillus acidoterrestris plate, but number of colonies varied in different beverages.
Three cloudy samples had a higher colony number over 103 CFU/mL and three clear samples had a lower colony number
below 103 CFU/mL. All samples inoculated with Alicyclobacillus sp. DSM 3922 could be distinguished from control by
E-tongue method. Excepted for mixed fruit juice and orange juice, other samples with Alicyclobacillus sp. XC-6 inoculation
could be grouped from the control by E-tongue method.

Key words: Alicyclobacillus sp., fruit juice beverage, electronic tongue, principal component analysis